BIOCHEMICAL AND GENETIC STUDIES WITH NITRATE REDUCTASE C-GENE MUTANTS OF ESCHERICHIA-COLI

被引:62
作者
GUEST, JR
机构
[1] Department of Microbiology, University of Sheffield
来源
MOLECULAR AND GENERAL GENETICS | 1969年 / 105卷 / 04期
关键词
D O I
10.1007/BF00277583
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Twenty-eight narC (chlC) mutants of Escherichia coli were isolated and characterised by their resistance to chlorate, inability to use nitrate as terminal electron acceptor and positive gas reaction. The extent of gas production by the majority of mutants was almost normal but quantitative differences ranging from 40 to 100% of wild-type activity were found. Biochemical studies showed that all the mutants lacked nitrate reductase, decreasing gas production was correlated with a simultaneous decrease in formate dehydrogenase activity and the lowest gas production was due to deficiencies in formate dehydrogenase and hydrogenase. The position of narC relative to other loci was determined as: purB ... hemA ... narC ... supIII,C ... galU ... attΦ80 ... tonB ... trp ... cysB by transduction analysis, and the mutant sites of 6 strains representing the complete range of gas reactions were clustered at this position. It is suggested that narC is the structural gene for nitrate reductase and the variations in phenotype may be due to polarity effects on neighbouring genes specifying components of the formate hydrogenlyase system. Transduction of narC by Φ80 could not be detected but an effect of galU- on phage P1kc susceptibility was demonstrated. © 1969 Springer-Verlag.
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页码:285 / &
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