PROPERTIES OF AFLATOXIN-DNA ADDUCTS FORMED BY PHOTOACTIVATION AND CHARACTERIZATION OF THE MAJOR PHOTOADDUCT AS AFLATOXIN-N7-GUANINE

被引：15

作者：

SHAULSKY, G

JOHNSON, RL

SHOCKCOR, JP

TAYLOR, LCE

STARK, AA

机构：

[1] TEL AVIV UNIV,DEPT BIOCHEM,IL-69978 TEL AVIV,ISRAEL

[2] BURROUGHS WELLCOME CO,RES TRIANGLE PK,NC 27709

来源：

CARCINOGENESIS | 1990年 / 11卷 / 04期

关键词：

D O I：

10.1093/carcin/11.4.519

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

DNA adducts were formed by microsomal and photoactivation, using nick-translated DNA labelled with 14C in each of the DNA bases [3H]AFB1 and [3H] AFB2. DNA adducts were analysed by HPLC of DNA hydrolysates, and were characterized as double labelled peaks with specific retention times. The only AF-DNA adducts which were detected in signincant amounts were guanine adducts, irrespective of the type of aflatoxin used or the mode of its activation. No stable adduct with adenine, cytcshe or thymine was detected. UV spectra, proton NMR spectroscopy and mass spectrometry are consistent with the notion that the major AFB1-DNA photoadduct is the N7-guanine adduct. This report provides direct evidence for the existence of ahtoxin photoadducts formed on DNA. © 1990 Oxford University Press.

引用

页码：519 / 527

页数：9

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