CLONING AND CHARACTERIZATION OF GENTAMICIN-RESISTANCE GENES FROM MICROMONOSPORA-PURPUREA AND MICROMONOSPORA-ROSEA

被引：40

作者：

KELEMEN, GH

CUNDLIFFE, E

FINANCSEK, I

机构：

[1] BIOCHIN BIOTECHNOL CO, H-1045 BUDAPEST, HUNGARY

[2] UNIV LEICESTER, LEICESTER BIOCTR, LEICESTER LE1 7RH, ENGLAND

来源：

GENE | 1991年 / 98卷 / 01期

关键词：

RECOMBINANT DNA; AMINOGLYCOSIDE-RESISTANCE; TRANSCRIPT MAPPING; RIBOSOMAL RNA METHYLASE;

D O I：

10.1016/0378-1119(91)90103-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aminoglycoside-resistance genes (grm) were cloned from a gentamicin producer Micromonospora purpurea and a sisomicin producer Micromonospora rosea. The nucleotide (nt) sequences of both genes were determined and the similarity between them was very high (90.4% identity). In either case, the transcription start point was localised to about 11 nt upstream from the likely translation start codons of grm, which is expressed as a polycistronic transcript. In studies to be reported elsewhere, it has been established that the M. purpurea grm gene encodes a ribosomal RNA methyltransferase. Here, we confirmed that the similarity of the two genes exists not only at the structural but also at the functional level.

引用

页码：53 / 60

页数：8

共 28 条

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