CANDIDIASIS VISUALIZED BY PROTEINASE-DIRECTED IMMUNOFLUORESCENCE

被引：38

作者：

RUCHEL, R ^{[1
]}

ZIMMERMANN, F ^{[1
]}

BONINGSTUTZER, B ^{[1
]}

HELMCHEN, U ^{[1
]}

机构：

[1] UNIV GOTTINGEN,DEPT PATHOL,W-3400 GOTTINGEN,GERMANY

来源：

VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY | 1991年 / 419卷 / 03期

关键词：

CANDIDIASIS; SECRETORY PROTEINASE; IMMUNOFLUORESCENCE;

D O I：

10.1007/BF01626348

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The secretory aspartic proteinases of Candida albicans and C. tropicalis are potential factors for virulence produced during infection. By indirect immunofluorescence, we have demonstrated proteinase antigen on elements of both species in deparaffinized tissue sections derived from clinical cases of mucosal and deep-seated candidiasis. Occasionally, we observed a halo of fluorescence in the close vicinity of candidal cells, which may reflect secretion of the enzyme. In kidneys, a ring of amorphous fluorescent material surrounding candidal colonies may illustrate alkaline denaturation of secreted enzyme within a pH gradient, which is generated by the fungus. Our findings support the view that candidal proteinase may be a diagnostically relevant antigen.

引用

页码：199 / 202

页数：4

共 24 条

[1]

BERGER U, 1988, MYCOSES S1, V31, P39

[2] EXPRESSION OF EXTRACELLULAR ACID PROTEINASE BY PROTEOLYTIC CANDIDA SPP DURING EXPERIMENTAL-INFECTION OF ORAL-MUCOSA [J].