TRANSPORT AND METABOLISM OF BROMOSULFOPHTHALEIN BY ISOLATED RAT-LIVER CELLS

被引：31

作者：

SCHWARZ, LR ^{[1
]}

SUMMER, KH ^{[1
]}

SCHWENK, M ^{[1
]}

机构：

[1] UNIV TUBINGEN,INST TOXIKOL,D-7400 TUBINGEN 1,FED REP GER

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb12932.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kinetics of transport and metabolism of bromosulfophthalein have been studied in isolated liver cells in a dose‐dependent manner obtaining the following results. The disposition of bromosulfophthalein in suspensions of isolated liver cells is similar to the turnover in the whole liver. The initial maximal rate of uptake of bromosulfophthalein is 2–3 times faster than intracellular conjugation with glutathione. Conjugation proceeds to an equilibrium between intracellular substrate (bromosulfophthalein) and product (bromosulfophthalein‐glutathione conjugate) which are both transiently accumulated in the cell. Formation of bromosulfophthaleinglutathione is accompanied by an equimolar decrease of glutathione. The bromosulfophthaleinglutathione conjugate is slowly released from the cells in an energy‐dependent and saturable transport process. The maximal velocity of excretion amounts to only 6% of the maximal velocity of uptake and to 20% of the maximal velocity of conjugation. Excretion, therefore, represents the slowest step in the overall turnover. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：617 / 622

页数：6

共 34 条

[1]

ARIAS IM, 1977, LIVER BILE, P157

[2] BINDING OF SULFOBROMOPHTHALEIN (BSP) SODIUM BY PLASMA ALBUMIN . ITS ROLE IN HEPATIC BSP EXTRACTION [J].

BAKER, KJ ;

BRADLEY, SE .

JOURNAL OF CLINICAL INVESTIGATION, 1966, 45 (02) :281-&

[3] CRITERIA OF VIABILITY OF ISOLATED LIVER-CELLS [J].

BAUR, H ;

KASPEREK, S ;

PFAFF, E .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1975, 356 (06) :827-838

[4]

BERNT E, 1970, METHODEN ENZYMATISCH, P1605

[5] HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS - A BIOCHEMICAL AND FINE STRUCTURAL STUDY [J].

BERRY, MN ;

FRIEND, DS .

JOURNAL OF CELL BIOLOGY, 1969, 43 (03) :506-+

[6]

BRAUER RW, 1949, J PHARMACOL EXP THER, V97, P358

[7] INTERACTION OF BROMOSULFOPHTHALEIN WITH MITOCHONDRIAL-MEMBRANES - UPTAKE OF BROMOSULFOPHTHALEIN AND EFFECT ON ANS-FLUORESCENCE [J].

BURR, R ;

SCHWENK, M ;

PFAFF, E .

BIOCHEMICAL PHARMACOLOGY, 1977, 26 (06) :457-460

[8] A LIVER ENZYME THAT CONJUGATES SULFOBROMOPHTHALEIN SODIUM WITH GLUTATHIONE [J].

COMBES, B ;

STAKELUM, GS .

JOURNAL OF CLINICAL INVESTIGATION, 1961, 40 (06) :981-&

[9] MECHANISMS OF HEPATIC BILE FORMATION [J].

FORKER, EL .

ANNUAL REVIEW OF PHYSIOLOGY, 1977, 39 :323-347

[10] EVIDENCE FOR EXISTENCE OF A CARRIER FOR BROMOSULFOPHTHALEIN IN LIVER-CELL PLASMA-MEMBRANE [J].

FREZZA, M ;

TIRIBELLI, C ;

PANFILI, E ;

SANDRI, G .

FEBS LETTERS, 1974, 38 (02) :125-128

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