ENHANCEMENT OF VARICELLA-ZOSTER VIRUS PLAQUING EFFICIENCY WITH AN AGAROSE OVERLAY MEDIUM

被引：9

作者：

KRAH, DL ^{[1
]}

SCHOFIELD, TL ^{[1
]}

PROVOST, PJ ^{[1
]}

机构：

[1] MERCK SHARP & DOHME LTD,DEPT BIOMETR RES,W POINT,PA 19486

来源：

JOURNAL OF VIROLOGICAL METHODS | 1990年 / 27卷 / 03期

关键词：

Agarose overlay plaque assay; Varicella-zoster virus plaque assay; Virus vaccine potency testing;

D O I：

10.1016/0166-0934(90)90100-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The infectivity titers of varicella-zoster virus (VZV) are routinely estimated by plaque production in cell culture. In this report, we show that plaque counts for VZV (strain Oka/Merck), in MRC-5 cell cultures, are significantly enhanced (54% average enhancement) by the use of an agarose overlay medium, as compared to a fluid overlay medium. Evidence also is presented that less variability (P < 0.05) in plaque counts occurs with the use of an agarose overlay medium. © 1990.

引用

页码：319 / 326

页数：8

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