RESTRICTION MAP, PARTIAL CLONING AND LOCALIZATION OF 9S AND 12S KINETOPLAST RNA GENES ON THE MAXICIRCLE COMPONENT OF THE KINETOPLAST DNA OF LEISHMANIA-TARENTOLAE

被引：36

作者：

MASUDA, H

SIMPSON, L

ROSENBLATT, H

SIMPSON, AM

机构：

[1] UNIV CALIF LOS ANGELES,DEPT BIOL,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,INST MOLEC BIOL,LOS ANGELES,CA 90024

来源：

GENE | 1979年 / 6卷 / 01期

关键词：

E; coli; hemoflagellate protozoa; pBR322; plasmid; Restriction endonucleases EcoRI; BamHI; HaeIII; SalI; BglII; HindIII;

D O I：

10.1016/0378-1119(79)90085-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a restriction map of the maxicircle component of the kinetoplast DNA of Leishmania tarentolae for the enzymes EcoRI, BamHI, HaeIII, HpaII, SalI, BglII and HindIII. the 9 and 12S kinetoplast RNAs were localized on this map. Two fragments of this maxicircle molecule were cloned in the bacterial plasmid, pBR322, including a 4.4 · 106 dalton EcoRI/BamHi fragment which contains the 9 and 12S RNA genes. © 1979.

引用

页码：51 / 73

页数：23

共 41 条

[1] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].