ULTRASTRUCTURAL IMMUNOLOCALIZATION OF ACTIN IN A FUNGUS

被引：66

作者：

BOURETT, TM ^{[1
]}

HOWARD, RJ ^{[1
]}

机构：

[1] DUPONT CO,DEPT CENT RES & DEV,POB 80402,WILMINGTON,DE 19880

来源：

PROTOPLASMA | 1991年 / 163卷 / 2-3期

关键词：

ACTIN; FREEZE SUBSTITUTION; FUNGI; HYPHAL TIP; IMMUNOCYTOCHEMISTRY; MAGNAPORTHE-GRISEA;

D O I：

10.1007/BF01323344

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have successfully localized fungal actin for the first time using immuno-electron microscopy and hyphal tips of the rice blast pathogen Magnaporthe grisea. Following ultrarapid freezing, samples were processed in a novel substitution fluid of 10% acrolein in anhydrous ethanol and embedded in LR White resin. A monoclonal anti-actin antibody, previously shown to recognize M. grisea actin, bound specifically to filasomes concentrated in the peripheral cytoplasm of subapical regions, and to the core-region of the Spitzenkorper.

引用

页码：199 / 202

页数：4

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