OXOLINIC ACID AND FLUMEQUINE IN FISH-TISSUES - VALIDATION OF AN HPLC METHOD - ANALYSIS OF MEDICATED FISH AND COMMERCIAL FISH SAMPLES

被引：21

作者：

DEGROODT, JM

DEBUKANSKI, BW

SREBRNIK, S

机构：

[1] Ministry of Public Health and Environment, B-1050 Brussels, 14, J. Wytsnam Street

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 08期

关键词：

D O I：

10.1080/10826079408013458

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes a simple method for residue analysis of oxolinic acid and flumequine in fish tissues by HPLC and fluorometric detection. The quinolones were extracted with ethyl acetate, purified with hexane and then analyzed using a RP-8 Lichrosorb reversed phase column. The method was validated with satisfying results. The high sensitivity of the fluorometric detection allowed to reach a quantification limit of 2 mu g oxolinic acid and 5 mu g flumequine/kg fish tissue and an absolute detection limit of 20 pg and 50 pg respectively. Medicated fish as well as commercial cultured fish samples were analyzed with the described method. Oxolinic acid and flumequine were detected in both of them.

引用

页码：1785 / 1794

页数：10

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