GENE-TRANSFER AND THE EXPRESSION OF A FOREIGN GENE IN-VIVO IN POSTMITOTIC NEURONS OF THE ADULT-RAT BRAIN USING THE HEMAGGLUTINATING VIRUS OF THE JAPAN-LIPOSOME METHOD

Neurons in the adult rat brain were transfected in vivo with a simple plasmid that harbored the gene for beta-galactosidase from Escherichia coli under control of a chicken beta-actin promoter by use of the hemagglutinating virus of Japan (HVJ) and liposomes. Cells that expressed beta-galactosidase were detected only in the target area of the central nervous system for 10 days by light microscopic analysis. Since electron microscopic analysis revealed that the products of the histochemical reaction were predominantly associated with the nuclear membrane and the endoplasmic reticulum of positive cells, it appeared that the products were translated endogenously and had not been entrapped by endocytosis. Furthermore, the products were observed in typical neuronal cells with a large, round, and pale nuclei, and with direct axe-somatic and axo-dendritic synaptic contacts. This report suggests the possibility of introducing functionally significant genes into neurons in targeted areas of the adult central nervous system.