SEQUENCE OF FIMBRIAL SUBUNIT-ENCODING GENES FROM VIRULENT AND BENIGN ISOLATES OF DICHELOBACTER-(BACTEROIDES)-NODOSUS

被引:7
作者
BILLINGTON, SJ [1 ]
ROOD, JI [1 ]
机构
[1] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA
关键词
RECOMBINANT DNA; OVINE PATHOGEN; FOOTROT; TWITCHING MOTILITY; ANTIGENICITY; PILIN;
D O I
10.1016/0378-1119(91)90042-A
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Both virulent and benign isolates of the ovine pathogen Dichelobacter (Bacteroides) nodosus produce polar fimbriae which have been implicated in twitching motility. The fimbrial subunit-encoding genes from two virulent and two benign serogroup-B isolates of D. nodosus were cloned and sequenced. Analysis of the deduced amino acid (aa) sequences of these subunits indicated the presence of substitutions that appeared to correlate with the virulence phenotype. However, these aa substitutions were located in variable regions of the protein where they are unlikely to alter the functional properties of the fimbriae. The aa sequences of the serogroup-B subunits had a very high level (91-95%) of similarity, particularly at the N terminus, where the conserved region extended up to aa 61. Specific aa substitutions within the subunit of one isolate may reflect its serotypic variation from the other serogroup-B subunits studied.
引用
收藏
页码:115 / 119
页数:5
相关论文
共 17 条
[1]   CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE GENE ENCODING THE STRUCTURAL SUBUNIT OF BACTEROIDES-NODOSUS FIMBRIAE [J].
ANDERSON, BJ ;
BILLS, MM ;
EGERTON, JR ;
MATTICK, JS .
JOURNAL OF BACTERIOLOGY, 1984, 160 (02) :748-754
[2]   MORPHOGENETIC EXPRESSION OF MORAXELLA-BOVIS FIMBRIAE (PILI) IN PSEUDOMONAS-AERUGINOSA [J].
BEARD, MKM ;
MATTICK, JS ;
MOORE, LJ ;
MOTT, MR ;
MARRS, CF ;
EGERTON, JR .
JOURNAL OF BACTERIOLOGY, 1990, 172 (05) :2601-2607
[3]  
Beveridge WIB, 1941, CSIR B, V140
[4]   IDENTIFICATION AND GENETIC-MAPPING OF PROTEINS ENCODED IN THE FIMBRIAL SUBUNIT GENE REGION OF BACTEROIDES-NODOSUS [J].
BILLINGTON, SJ ;
MITCHISON, M ;
ADLER, B ;
ROOD, JI .
VETERINARY MICROBIOLOGY, 1990, 22 (01) :53-68
[5]  
BOULOS S, 1986, FEMS MICROBIOL LETT, V33, P73, DOI 10.1111/j.1574-6968.1986.tb01215.x
[6]   AN ANALYSIS OF THE ORGANIZATION AND EVOLUTION OF TYPE-4 FIMBRIAL (MEPHE) SUBUNIT PROTEINS [J].
DALRYMPLE, B ;
MATTICK, JS .
JOURNAL OF MOLECULAR EVOLUTION, 1987, 25 (03) :261-269
[7]   MOTILITY IN RELATION TO VIRULENCE OF BACTEROIDES-NODOSUS [J].
DEPIAZZI, LJ ;
RICHARDS, RB .
VETERINARY MICROBIOLOGY, 1985, 10 (02) :107-116
[8]  
DEPIAZZI LJ, 1990, VET MICROBIOL
[9]   TRANSFER OF KINGELLA-INDOLOGENES (SNELL AND LAPAGE 1976) TO THE GENUS SUTTONELLA GEN-NOV AS SUTTONELLA-INDOLOGENES COMB-NOV - TRANSFER OF BACTEROIDES-NODOSUS (BEVERIDGE 1941) TO THE GENUS DICHELOBACTER GEN-NOV AS DICHELOBACTER-NODOSUS COMB-NOV - AND ASSIGNMENT OF THE GENERA CARDIOBACTERIUM, DICHELOBACTER, AND SUTTONELLA TO CARDIOBACTERIACEAE FAM NOV IN THE GAMMA-DIVISION OF PROTEOBACTERIA ON THE BASIS OF 16S RIBOSOMAL-RNA SEQUENCE COMPARISONS [J].
DEWHIRST, FE ;
PASTER, BJ ;
LAFONTAINE, S ;
ROOD, JI .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1990, 40 (04) :426-433