ABERRATION COMPENSATION IN CONFOCAL MICROSCOPY

被引:96
作者
SHEPPARD, CJR
GU, M
机构
[1] Department of Physical Optics, School of Physics, University of Sydney, Sydney, NSW
来源
APPLIED OPTICS | 1991年 / 30卷 / 25期
关键词
D O I
10.1364/AO.30.003563
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
In confocal microscopy, spherical aberration is introduced when one is focusing deep within the specimen. This can be compensated for by altering the effective tube length at which the objective is operated. The limitations of this approach are investigated.
引用
收藏
页码:3563 / 3568
页数:6
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