DETECTION OF ANTIBODIES TO OVINE LENTIVIRUS USING A RECOMBINANT ANTIGEN DERIVED FROM THE ENV GENE

被引：27

作者：

KWANG, J ^{[1
]}

CUTLIP, R ^{[1
]}

机构：

[1] USDA ARS,NATL ANIM DIS CTR,AMES,IA 50010

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)80295-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Western blot assay was performed to characterize antibodies to the transmembrane glycoprotein (TGP) of ovine progressive pneumonia virus (OPPV) by using glutathione-S-transferase-TGP (GST-TGP) protein. The GST-TGP protein was efficiently expressed in Escherichia coli (E. coli) and was highly immunoreactive in the Western blot assay. This assay detected antibodies in 97% (103/106) of the sera from agarose gel immunodiffusion (AGID) positive OPP animals. Like human AIDS patients, antibodies to TGP appear to be one of the major serological markers in OPP infected animals. © 1992 Academic Press, Inc.

引用

页码：1040 / 1046

页数：7

共 23 条

[1] THE GP135 OF CAPRINE ARTHRITIS ENCEPHALITIS-VIRUS AFFORDS GREATER SENSITIVITY THAN THE P-28 IN IMMUNODIFFUSION SEROLOGY [J].