KINETIC-STUDIES AND MODELING OF A SELF-REPLICATING SYSTEM

被引：131

作者：

NOWICK, JS ^{[1
]}

FENG, Q ^{[1
]}

TJIVIKUA, T ^{[1
]}

BALLESTER, P ^{[1
]}

REBEK, J ^{[1
]}

机构：

[1] MIT,DEPT CHEM,CAMBRIDGE,MA 02139

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1991年 / 113卷 / 23期

关键词：

D O I：

10.1021/ja00023a036

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The design, synthesis, and study of a molecular template, 11a, that catalyzes its own formation from simpler components, 9 and 10d, is described. Autocatalysis is shown by the following: (1) the increase in rate of reaction of 9 and 10d in the presence of lla; (2) the reduction in rate of reaction in the presence of 2,6-bis(acetylamino)pyridine inhibitor; and (3) the reduction in rate of reaction when a component incapable of template behavior, 10h, is substituted for 10d. Three kinetic pathways are elucidated: (1) the background bimolecular reaction (eq 6); (2) the preassociative mechanism (eq 7); and (3) the template termolecular process (eq 8). A kinetic model for the replication process is introduced, and equilibrium and rate constants are determined. Predictions for the shapes of the product growth curves are made.

引用

收藏

页码：8831 / 8839

页数：9

相关论文

共 25 条

[1]

[Anonymous], 1946, CHEM ENG NEWS, DOI DOI 10.1021/CEN-V024N010.P1375

[2] MOLECULAR RECOGNITION WITH CONVERGENT FUNCTIONAL-GROUPS .6. SYNTHETIC AND STRUCTURAL STUDIES WITH A MODEL RECEPTOR FOR NUCLEIC-ACID COMPONENTS [J].

ASKEW, B ;

BALLESTER, P ;

BUHR, C ;

JEONG, KS ;

JONES, S ;

PARRIS, K ;

WILLIAMS, K ;

REBEK, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (03) :1082-1090

[3] SELF-REPLICATING REVERSE MICELLES AND CHEMICAL AUTOPOIESIS [J].

BACHMANN, PA ;

WALDE, P ;

LUISI, PL ;

LANG, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (22) :8200-8201

[4] STRONG BINDING OF DITOPIC SUBSTRATES BY A DOUBLY LINKED OCCLUSIVE C1 CLAMSHELL AS DISTINGUISHED FROM AN AVERSIVE C2 LOVESEAT CYCLODEXTRIN [J].

BRESLOW, R ;

CHUNG, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (26) :9659-9660

[5] A MULTISUBUNIT RIBOZYME THAT IS A CATALYST OF AND TEMPLATE FOR COMPLEMENTARY STRAND RNA-SYNTHESIS [J].

DOUDNA, JA ;

COUTURE, S ;

SZOSTAK, JW .

SCIENCE, 1991, 251 (5001) :1605-1608

[6] RNA-CATALYZED SYNTHESIS OF COMPLEMENTARY-STRAND RNA [J].

DOUDNA, JA ;

SZOSTAK, JW .

NATURE, 1989, 339 (6225) :519-522

[7] GENERATION OF A LARGE COMBINATORIAL LIBRARY OF THE IMMUNOGLOBULIN REPERTOIRE IN PHAGE-LAMBDA [J].

HUSE, WD ;

SASTRY, L ;

IVERSON, SA ;

KANG, AS ;

ALTINGMEES, M ;

BURTON, DR ;

BENKOVIC, SJ ;

LERNER, RA .

SCIENCE, 1989, 246 (4935) :1275-1281

[8] BISUBSTRATE REACTION TEMPLATES - EXAMINATION OF THE CONSEQUENCES OF IDENTICAL VERSUS DIFFERENT BINDING-SITES [J].

KELLY, TR ;

BRIDGER, GJ ;

ZHAO, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (22) :8024-8034

[9] SYNTHESIS AND CONFORMATIONAL-ANALYSIS OF CIS,CIS-1,3,5-TRIMETHYLCYCLOHEXANE-1,3,5-TRICARBOXYLIC ACID [J].

KEMP, DS ;

PETRAKIS, KS .

JOURNAL OF ORGANIC CHEMISTRY, 1981, 46 (25) :5140-5143

[10] PEPTIDE-SYNTHESIS BY PRIOR THIOL CAPTURE .2. DESIGN OF TEMPLATES FOR INTRAMOLECULAR O,N-ACYL TRANSFER - 4,6-DISUBSTITUTED DIBENZOFURANS AS OPTIMAL SPACING ELEMENTS [J].

KEMP, DS ;

GALAKATOS, NG ;

BOWEN, B ;

TAN, K .

JOURNAL OF ORGANIC CHEMISTRY, 1986, 51 (10) :1829-1838