STABILITY ANALYSIS OF THE LACTOCOCCUS-LACTIS DRC1 LACTOSE PLASMID USING PULSED-FIELD GEL-ELECTROPHORESIS

被引：7

作者：

WARD, AC

HILLIER, AJ

DAVIDSON, BE

POWELL, IB

机构：

[1] CSIRO,DIV FOOD PROC,DAIRY RES LAB,POB 20,HIGHETT,VIC 3190,AUSTRALIA

[2] UNIV MELBOURNE,RUSSELL GRIMWADE SCH BIOCHEM,PARKVILLE,VIC 3052,AUSTRALIA

来源：

PLASMID | 1993年 / 29卷 / 01期

关键词：

D O I：

10.1006/plas.1993.1009

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Pulsed-field agarose gel electrophoresis of Sma1 digests of genomic DNA was used to examine lactose plasmid copy number and stability in Lactococcus lactis. In L. lactis strain DRC1, the plasmid was found to exist as a single-copy plasmid. Transconjugants of strain HID113 carrying this plasmid were unstable. Variants were isolated with improved phenotypic stability resulting from improved maintenance of the lactose plasmid or from integration of part of the plasmid into the lactococcal chromosome. © 1993 Academic Press, Inc.

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页码：70 / 73

页数：4

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