A SIMPLE METHOD FOR IMMUNOFLUORESCENCE STAINING OF TRACHEAL ORGAN-CULTURES FOR THE RAPID IDENTIFICATION OF INFECTIOUS-BRONCHITIS VIRUS

被引：18

作者：

BHATTACHARJEE, PS

NAYLOR, CJ

JONES, RC

机构：

[1] University of Liverpool, Department of Veterinary Pathology, Leahurst, Neston, South Wirral

[2] AFRC Institute for Animal Health Compton, Newbury

来源：

AVIAN PATHOLOGY | 1994年 / 23卷 / 03期

关键词：

D O I：

10.1080/03079459408419017

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A method is described for the simple application of immunofluorescence (IF) staining for the identification of infectious bronchitis virus (IBV) in tracheal organ cultures (TOC). It involves the application of antiserum and fluorescent antiglobulin to unfixed TOC. In TOC inoculated with high titres of virus, specific fluorescence was observed in less than 24 h. IF staining almost always occurred before ciliostasis. Following experimental infection of chicks with IBV, virus could sometimes be detected in material horn tracheal or cloacal swabs within 24 h of inoculation of TOC.

引用

页码：471 / 480

页数：10

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