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CLONING AND COMPLETE SEQUENCE OF THE DNA POLYMERASE-ENCODING GENE (BSTPOLI) AND CHARACTERIZATION OF THE KLENOW-LIKE FRAGMENT FROM BACILLUS-STEAROTHERMOPHILUS

被引：19
作者：
PHANG, SM
TEO, CY
LO, E
WONG, VWT
机构：
[1] NATL UNIV SINGAPORE,DEPT ZOOL,GENE CLONING LAB,SINGAPORE 0511,SINGAPORE
[2] MICROSTATE SEPARAT PTE LTD,SINGAPORE 2057,SINGAPORE
来源：
GENE | 1995年 / 163卷 / 01期
关键词：
DNA SEQUENCING; GENOMIC LIBRARY; HOMOLOGIES; RECOMBINANT;
D O I：
10.1016/0378-1119(95)00387-L
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme.
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页码：65 / 68
页数：4
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