ADENOVIRUS 5 DNA SEQUENCES PRESENT AND RNA SEQUENCES TRANSCRIBED IN TRANSFORMED HUMAN-EMBRYO KIDNEY-CELLS (HEK-AD-5 OR 293)

被引：152

作者：

AIELLO, L ^{[1
]}

GUILFOYLE, R ^{[1
]}

HUEBNER, K ^{[1
]}

WEINMANN, R ^{[1
]}

机构：

[1] WISTAR INST ANAT & BIOL,36TH ST SPRUCE,PHILADELPHIA,PA 19104

来源：

VIROLOGY | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1016/0042-6822(79)90476-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The viral DNA sequences present in the human embryo kidney cell line (293) transformed from sheared adenovirus 5 fragments (F. L. Graham, P. S. Abrahams, C. Mulder, H. L. Heijneker, S. 0. Warnaar, F. A. deVries, W. Fiers, and A. J. van der Eb 1974, Cold Spring Harbor Symp. Quant. Biol.39, 637-650) were analyzed. By renaturation kinetics of transformed cell DNA and labeled adenovirus 5 restriction enzyme fragments obtained with HindIII we determined that there are four to five copies per cell of the left 12% of the viral genome and approximately one copy per cell of 9% of the right end of the viral genome. Analysis of the RNA sequences transcribed in nuclei isolated from this cell line indicated that only transcripts from the sequences of the left end were detected. The expression of these viral sequences was mediated by DNA-dependent RNA polymerase II. Analysis of the viral DNA from 293 cells using restriction enzymes reveals that the viral DNA is integrated and located at two sites on the cellular genome. This simple arrangement is stable through several cell passages. © 1979.

引用

页码：460 / 469

页数：10

共 26 条

[1] TRANSCRIPTION OF VIRAL GENES BY RNA POLYMERASE-II IN NUCLEI ISOLATED FROM ADENOVIRUS 2 TRANSFORMED-CELLS [J].

BITTER, GA ;

ROEDER, RG .

BIOCHEMISTRY, 1978, 17 (11) :2198-2205

[2] ARRANGEMENT OF SIMIAN-VIRUS 40 SEQUENCES IN DNA OF TRANSFORMED-CELLS [J].

BOTCHAN, M ;

TOPP, W ;

SAMBROOK, J .

CELL, 1976, 9 (02) :269-287

[3] REPEATED SEQUENCES IN DNA [J].

BRITTEN, RJ ;

KOHNE, DE .

SCIENCE, 1968, 161 (3841) :529-&

[4] ANALYSIS OF EARLY ADENOVIRUS-2 RNA USING ECO R.R1 VIRAL DNA FRAGMENTS [J].

CRAIG, EA ;

ZIMMER, S ;

RASKAS, HJ .

JOURNAL OF VIROLOGY, 1975, 15 (05) :1202-1213

[5] UNIDIRECTIONAL LOSS OF HUMAN CHROMOSOMES IN RAT-HUMAN HYBRIDS [J].

CROCE, CM ;

KIEBA, I ;

KOPROWSKI, H .

EXPERIMENTAL CELL RESEARCH, 1973, 79 (02) :461-463

[6] A MEMBRANE-FILTER TECHNIQUE FOR DETECTION OF COMPLEMENTARY DNA [J].

DENHARDT, DT .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1966, 23 (05) :641-&

[7] VIRAL NUCLEIC-ACID SEQUENCES IN TRANSFORMED-CELLS .4. STUDY OF SEQUENCES OF ADENOVIRUS 5 DNA AND RNA IN 4 LINES OF ADENOVIRUS 5-TRANSFORMED RODENT CELLS USING SPECIFIC FRAGMENTS OF VIRAL GENOME [J].

FLINT, SJ ;

SAMBROOK, J ;

WILLIAMS, JF ;

SHARP, PA .

VIROLOGY, 1976, 72 (02) :456-470

[8] VIRAL DNA IN TRANSFORMED-CELLS .2. STUDY OF SEQUENCES OF ADENOVIRUS-2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF VIRAL GENOME [J].

GALLIMORE, PH ;

SHARP, PA ;

SAMBROOK, J .

JOURNAL OF MOLECULAR BIOLOGY, 1974, 89 (01) :49-72

[9] STUDIES ON INVITRO TRANSFORMATION BY DNA AND DNA FRAGMENTS OF HUMAN ADENOVIRUSES AND SIMIAN VIRUS 40 [J].

GRAHAM, FL ;

ABRAHAMS, PJ ;

MULDER, C ;

HEIJNEKER, HL ;

WARNAAR, SO ;

DEVRIES, FAJ ;

FIERS, W ;

VANDEREB, AJ .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1974, 39 :637-650

[10] CHARACTERISTICS OF A HUMAN CELL LINE TRANSFORMED BY DNA FROM HUMAN ADENOVIRUS TYPE-5 [J].

GRAHAM, FL ;

SMILEY, J ;

RUSSELL, WC ;

NAIRN, R .

JOURNAL OF GENERAL VIROLOGY, 1977, 36 (JUL) :59-72

← 1 2 3 →