HUMANIZED ANTIBODIES FOR ANTIVIRAL THERAPY

被引：106

作者：

CO, MS ^{[1
]}

DESCHAMPS, M ^{[1
]}

WHITLEY, RJ ^{[1
]}

QUEEN, C ^{[1
]}

机构：

[1] UNIV ALABAMA, DEPT PEDIAT, BIRMINGHAM, AL 35294 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 07期

关键词：

HERPES SIMPLEX VIRUS; COMPUTER MODELING;

D O I：

10.1073/pnas.88.7.2869

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Antibody therapy holds great promise for the treatment of cancer, autoimmune disorders, and viral infections. Murine monoclonal antibodies are relatively easy to produce but are severely restricted for therapeutic use by their immunogenicity in humans. Production of human monoclonal antibodies has been problematic. Humanized antibodies can be generated by introducing the six hypervariable regions from the heavy and light chains of a murine antibody into a human framework sequence and combining it with human constant regions. We humanized, with the aid of computer modeling, two murine monoclonal antibodies against herpes simplex virus gB and gD glycoproteins. The binding, virus neutralization, and cell protection results all indicate that both humanized antibodies have retained the binding activities and the biological properties of the murine monoclonal antibodies.

引用

页码：2869 / 2873

页数：5

共 34 条

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