CHROMOSOMAL LOCALIZATION OF INTERGENOMIC RFLP LOCI IN HEXAPLOID WHEAT

被引：24

作者：

DEVEY, ME ^{[1
]}

HART, GE ^{[1
]}

机构：

[1] TEXAS A&M UNIV SYST,DEPT SOIL & CROP SCI,COLL STN,TX 77843

来源：

GENOME | 1993年 / 36卷 / 05期

关键词：

ANEUPLOIDS; GENETIC MAPPING;

D O I：

10.1139/g93-120

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hybridization of radiolabeled wheat DNA probes to genomic DNA digests of compensating nullisomictetrasomic lines and ditelosomic lines of hexaploid wheat (Triticum aestivum L. cv. Chinese Spring) can be used to identify intergenomic RFLPs. Sixty-three PstI/BamHI genomic DNA probes and eight cDNA probes were used to determine the chromosomal locations of 223 DNA fragments that define a minimum of 189 RFLP loci. Eighty-four percent of the genomic DNA clones hybridize to fragments located in homoeologous chromosomes and 16% hybridize to fragments located in one chromosome only or to fragments located in nonhomoeologous chromosomes. All of the cDNA probes hybridize to fragments located in homoeologous chromosomes.

引用

页码：913 / 918

页数：6

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