REGULATORY CIRCUITRY CONTROLLING LUMINESCENCE AUTOINDUCTION IN VIBRIO-FISCHERI

被引:37
作者
ULITZUR, S
DUNLAP, PV
机构
[1] TECHNION ISRAEL INST TECHNOL,DEPT FOOD ENGN & BIOTECHNOL,IL-32000 HAIFA,ISRAEL
[2] WOODS HOLE OCEANOG INST,DEPT BIOL,WOODS HOLE,MA 02543
关键词
D O I
10.1111/j.1751-1097.1995.tb08709.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Luminescence in the marine symbiotic bacterium Vibrio fischeri is subject to control by autoinduction, a regulatory mechanism that activates light production at high population density and suppresses light production at low population density. Several genetic, physiological and environmental factors contribute to autoinduction. Primary among these are a self-produced, membrane-permeable compound, N-3-oxohexanoyl-L-homoserine lactone, called autoinducer, which accumulates in a population density-dependent manner during growth of V. fischeri, and a transcriptional activator protein, LuxR, which with autoinducer activates transcription of the luminescence (lux) genes (luxICDABEG; encoding proteins for autoinducer synthesis and light production). Additional genetically defined regulatory elements involved in autoinduction include 3':5'-cyclic AMP (cAMP), which via cAMP receptor protein activates transcription of luxR, and the GroESL proteins, which stabilize LuxR in its active form. Evidence exists also for the involvement of LexA protein, for a second autoinducer, N-octanoyl-L-homoserine lactone, and under anaerobic conditions for Fnr protein. Besides these regulatory elements, nutrient limitation, presence of glucose, availability of iron and oxygen, temperature, salts and an autoinducer-LuxR protein-independent modulation also contribute to the autoinduction phenomenon. The multiplicity of genetic, physiological and environmental factors indicates that luminescence autoinduction is mediated by a complex regulatory circuitry, one that is highly integrated with and responsive to the physiological and ecological status of the cells. Long thought to be unique to V. fischeri and certain closely related marine luminous bacteria, luminescence autoinduction is now viewed as a model for understanding population density-responsive control of gene expression in a wide variety of terrestrial and marine bacteria in which N-acyl-L-homoserine lactones and homologs of LuxI and LuxR recently have been found.
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页码:625 / 632
页数:8
相关论文
共 87 条
[1]   FORMATION OF THE LUXR PROTEIN IN THE VIBRIO-FISCHERI-LUX SYSTEM IS CONTROLLED BY HTPR THROUGH THE GROESL PROTEINS [J].
ADAR, YY ;
SIMAAN, M ;
ULITZUR, S .
JOURNAL OF BACTERIOLOGY, 1992, 174 (22) :7138-7143
[2]   GROESL PROTEINS FACILITATE BINDING OF EXTERNALLY ADDED INDUCER BY LUXR PROTEIN-CONTAINING ESCHERICHIA-COLI-CELLS [J].
ADAR, YY ;
ULITZUR, S .
JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1993, 8 (05) :261-266
[3]   THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE LUX REGULON OF VIBRIO-FISCHERI AND THE LUXABN REGION OF PHOTOBACTERIUM-LEIOGNATHI AND THE MECHANISM OF CONTROL OF BACTERIAL BIOLUMINESCENCE [J].
BALDWIN, TO ;
DEVINE, JH ;
HECKEL, RC ;
LIN, JW ;
SHADEL, GS .
JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1989, 4 (01) :326-341
[4]   MULTIPLE SIGNALING SYSTEMS CONTROLLING EXPRESSION OF LUMINESCENCE IN VIBRIO-HARVEYI - SEQUENCE AND FUNCTION OF GENES ENCODING A 2ND SENSORY PATHWAY [J].
BASSLER, BL ;
WRIGHT, M ;
SILVERMAN, MR .
MOLECULAR MICROBIOLOGY, 1994, 13 (02) :273-286
[5]  
Baumann P., 1981, PROKARYOTES, P1302
[6]   FUNCTIONAL IDENTIFICATION OF THE FATTY-ACID REDUCTASE COMPONENTS ENCODED IN THE LUMINESCENCE OPERON OF VIBRIO-FISCHERI [J].
BOYLAN, M ;
GRAHAM, AF ;
MEIGHEN, EA .
JOURNAL OF BACTERIOLOGY, 1985, 163 (03) :1186-1190
[7]   LUX C, D AND E GENES OF THE VIBRIO-FISCHERI LUMINESCENCE OPERON CODE FOR THE REDUCTASE, TRANSFERASE, AND SYNTHETASE ENZYMES INVOLVED IN ALDEHYDE BIOSYNTHESIS [J].
BOYLAN, M ;
MIYAMOTO, C ;
WALL, L ;
GRAHAM, A ;
MEIGHEN, E .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1989, 49 (05) :681-688
[8]  
CAO JG, 1989, J BIOL CHEM, V264, P21670
[9]   ISOLATION AND CHARACTERIZATION OF A CYCLIC-AMP RECEPTOR PROTEIN FROM LUMINOUS VIBRIO-HARVEYI CELLS [J].
CHEN, PF ;
TU, SC ;
HAGAG, N ;
WU, FYH ;
WU, CW .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 241 (02) :425-431
[10]  
Choi S. H., 1992, Molecular Marine Biology and Biotechnology, V1, P408