EFFICIENT MECHANICAL INOCULATION OF TURNIP YELLOW MOSAIC-VIRUS USING SMALL VOLUMES OF INOCULUM

被引：4

作者：

FRASER, L ^{[1
]}

MATTHEWS, REF ^{[1
]}

机构：

[1] UNIV AUCKLAND, DEPT CELL BIOL, AUCKLAND, NEW ZEALAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1979年 / 44卷 / AUG期

关键词：

D O I：

10.1099/0022-1317-44-2-565

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

When purified turnip yellow mosaic virus was inoculated mechanically on to Chinese cabbage leaves, using known numbers of virus particles in 0.1-1.0 .mu.l volumes of inoculum, as few as 10-30 particles were required to produce a single local lesion. A simple procedure is described for obtaining local lesions from the virus in single infected protoplasts.

引用

页码：565 / 568

页数：4

共 14 条

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[2] GIBBS A.J., 1976, PLANT VIROLOGY PRINC
[3] QUANTITY OF MICROINJECTED TOBACCO MOSAIC-VIRUS REQUIRED FOR INFECTION OF SINGLE CULTURED TOBACCO CELLS
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[4] INFECTION OF COWPEA MESOPHYLL PROTOPLASTS WITH COWPEA MOSAIC-VIRUS
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[J]. VIROLOGY, 1975, 64 (02) : 308 - 318
[5] UPTAKE OF TOBACCO RATTLE VIRUS BY TOBACCO PROTOPLASTS, AND EFFECT OF PHOSPHATE ON INFECTION
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ROBINSON, DJ
HARRISON, BD
HUTCHESON, AM
[J]. JOURNAL OF GENERAL VIROLOGY, 1976, 30 (MAR) : 287 - 298
[6] LURIA SE, 1978, GENERAL VIROLOGY
[7] MATTHEWS R.E.F., 1970, Plant virology
[8] LIGHT-INDUCED STRUCTURAL-CHANGE IN CHLOROPLASTS OF CHINESE CABBAGE CELLS INFECTED WITH TURNIP YELLOW MOSAIC-VIRUS
MATTHEWS, REF
SARKAR, S
[J]. JOURNAL OF GENERAL VIROLOGY, 1976, 33 (DEC) : 435 - 446
[9] MATTHEWS REF, 1977, ATLAS INSECT PLANT V, P347
[10] DIRECT ESTIMATE OF NUMBER OF COWPEA CHLOROTIC MOTTLE VIRUS-PARTICLES ABSORBED BY TOBACCO PROTOPLASTS THAT BECOME INFECTED
MOTOYOSHI, F
BANCROFT, JB
WATTS, JW
[J]. JOURNAL OF GENERAL VIROLOGY, 1973, 21 (OCT) : 159 - 161

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