QUANTITATION OF SERUM TOBRAMYCIN CONCENTRATION USING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

被引：18

作者：

MAITRA, SK

YOSHIKAWA, TT

HANSEN, JL

SCHOTZ, MC

GUZE, LB

机构：

[1] HARBOR GEN HOSP,DEPT MED,DIV INFECT DIS,TORRANCE,CA 90509

[2] VET ADM WADSWORTH HOSP CTR,RES SERV,LOS ANGELES,CA 90073

[3] SCH MED LOS ANGELES,DEPT MED,LOS ANGELES,CA

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 1979年 / 71卷 / 04期

关键词：

D O I：

10.1093/ajcp/71.4.428

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A high-pressure liquid chromatography method for the quantitative determination of tobramycin in serum is described. The antibiotic was separated from serum by chromatography on a silica gel column. The adsorbed antibiotic was derivatized with o-phthaladehyde, and then eluted with isopropanol. The derivatized tobramycin was separated by reverse-phase chromatography and quantitated by fluorometry. Serum concentrations as low as 0.5 μg/ml could be accurately measured. A linear response for serum samples containing tobramycin ranging from 0 to 20 μg/ml was obtained. Other antibiotics, including various aminoglycosides, did not interfere with the tobramycin assay. Comparison with a standard microbiologic assay gave a correlation coefficient of 0.99. This chemical assay is sensitive, precise, specific, and can be performed in 30 minutes.

引用

页码：428 / 432

页数：5

共 10 条

[1] ORTHO-PHTHALALDEHYDE - FLUOROGENIC DETECTION OF PRIMARY AMINES IN PICOMOLE RANGE - COMPARISON WITH FLUORESCAMINE AND NINHYDRIN [J].