CONCENTRATION OF SERUM LAMININ AND TYPE-IV COLLAGEN IN LIVER-DISEASES ASSAYED BY A SANDWICH ENZYME-IMMUNOASSAY USING MONOCLONAL-ANTIBODIES

被引：17

作者：

YOKOYA, Y

IWATA, K

MURAGAKI, Y

SHIOTA, C

MORIMOTO, Y

INOUE, M

ITOH, H

NISHIOKA, S

OOSHIMA, A

机构：

[1] WAKAYAMA MED COLL, DIV INTERNAL MED 2, WAKAYAMA, JAPAN

[2] FUJI CHEM IND CO LTD, TAKAOKA, TOYAMA, JAPAN

来源：

CLINICA CHIMICA ACTA | 1992年 / 210卷 / 1-2期

关键词：

SANDWICH ENZYME-IMMUNOASSAY; HUMAN LAMININ; HUMAN TYPE-IV COLLAGEN; LIVER DISEASE; MONOCLONAL ANTIBODY;

D O I：

10.1016/0009-8981(92)90049-V

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Serum laminin (P1 fragment) and type IV collagen levels were determined in patients with hepatic disorders. The method was based on a sandwich enzyme-immunoassay using two monoclonal antibodies that recognize different epitopes of either laminin or type IV collagen molecule. Laminin and type IV collagen levels in the serum of patients with chronic hepatic disorders were higher as compared with those in healthy control subjects, with the increment of serum type IV collagen being far greater than that of laminin. Since type IV collagen and laminin are major basement membrane components, it is suggested that the higher levels of these peptides may reflect a so-called capillarization of the perisinusoidal wall encountered in hepatic fibrogenesis. The assay system used in this experiment is simple and sensitive and can be applied to clinical evaluation of hepatic fibrosis.

引用

页码：109 / 118

页数：10

共 23 条

[1] BASEMENT-MEMBRANE PRODUCTION BY HEPATOCYTES IN CHRONIC LIVER-DISEASE [J].