A HIGH-THROUGHPUT PLASMID DNA PREPARATION METHOD

被引：15

作者：

HUANG, GM

WANG, K

KUO, CL

PAEPER, B

HOOD, L

机构：

[1] Department of Molecular Biotechnology, University of Washington School of Medicine, Seattle, WA 98195

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 223卷 / 01期

关键词：

96-Well format - Cost effective - Genome projects - High throughput - High-throughput method - Microfilters - Preparation method - Restriction digestion;

D O I：

10.1006/abio.1994.1542

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Genome projects require a high-throughput method for DNA template preparation, which traditional protocols can rarely provide. We introduce here a new protocol for plasmid and cosmid DNA preparation based on alkaline denaturation in a 96-well format. The essential improvement made in this protocol on previous 96-well preparation includes the purification by Pro-Cipitate in 96-well microfilters. The yield and quality of DNA are sufficient for restriction digestion, radioactive sequencing, and automated fluorescent sequencing. It is also significantly more cost-effective than several of the commercial mini-prep kits. (C) 1994 Academic Press, Inc.

引用

页码：35 / 38

页数：4