MICROPLATE IMMUNOENZYME ASSAY FOR ANTI-INFLUENZA ANTIBODIES

被引：37

作者：

LAMBRE, C ^{[1
]}

KASTURI, KN ^{[1
]}

机构：

[1] INST PASTEUR, UNITE ECOL VIRALE, F-75724 PARIS, FRANCE

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 26卷 / 01期

关键词：

D O I：

10.1016/0022-1759(79)90041-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase is described for the detection and quantitation of anti-influenza virus antibodies. Compared with complement fixation and hemagglutination inhibition tests, ELISA is far superior with respect to sensitivity and reliability. Non-specific viral inhibitors present in sera do not affect the titer in ELISA. Its sensitivity, close to that of radioimmunoassay permits detection of small amounts of antibodies in pulmonary secretions and supernatants from in vitro spleen cell cultures. © 1979.

引用

页码：61 / 67

页数：7

共 16 条

[1] ALFORD RH, 1967, J IMMUNOL, V98, P724
[2] AVRAMEAS S, 1971, CR ACAD SCI D NAT, V273, P2705
[3] BISHAI FR, 1977, LANCET, V2, P696
[4] BRACIALE TJ, 1976, J IMMUNOL, V116, P827
[5] ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) QUANTITATIVE ASSAY OF IMMUNOGLOBULIN-G
ENGVALL, E
PERLMANN, P
[J]. IMMUNOCHEMISTRY, 1971, 8 (09): : 871 - &
[6] FAZEKAS DeST. GROTH S., 1966, J EXP MED, V124, P331
[7] JENSEN KE, 1961, AM REV RESPIR DIS, V83, P120
[8] PRODUCTION OF ANTIBODIES AGAINST INFLUENZA-VIRUS BY SOMATIC-CELL HYBRIDS BETWEEN MOUSE MYELOMA AND PRIMED SPLEEN-CELLS
KOPROWSKI, H
GERHARD, W
CROCE, CM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (07) : 2985 - 2988
[9] SOLID-PHASE ANTIBODY ASSAY BY MEANS OF ENZYME CONJUGATED TO ANTI-IMMUNOGLOBULIN
LEINIKKI, P
PASSILA, S
[J]. JOURNAL OF CLINICAL PATHOLOGY, 1976, 29 (12) : 1116 - 1120
[10] LOCAL HEMOLYSIS PLAQUE ASSAY USING A NEW METHOD OF COUPLING ANTIGENS ON SHEEP ERYTHROCYTES BY GLUTARALDEHYDE
LEMIEUX, S
AVRAMEAS, S
BUSSARD, AE
[J]. IMMUNOCHEMISTRY, 1974, 11 (05): : 261 - 269

← 1 2 →