THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENE CODING FOR BOTULINUM TYPE-C1 TOXIN IN THE C-ST PHAGE GENOME

被引：58

作者：

KIMURA, K

FUJII, N

TSUZUKI, K

MURAKAMI, T

INDOH, T

YOKOSAWA, N

TAKESHI, K

SYUTO, B

OGUMA, K

机构：

[1] SAPPORO MED COLL,DEPT MICROBIOL,SAPPORO,HOKKAIDO 060,JAPAN

[2] HOKKAIDO UNIV,FAC VET MED,DEPT BIOCHEM,SAPPORO,HOKKAIDO 060,JAPAN

[3] HOKKAIDO INST PUBL HLTH,DEPT EPIDEMIOL,SAPPORO 060,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90828-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two DNA fragments, 3 kbp and 7.8kbp, which encode the type C1 botulinum neurotoxin gene, were obtained from toxigenic bacteriophage DNA by treatment with a restriction enzyme. They were cloned into the plasmid vectors for nucleotide sequence determination. The nucleotide sequence contained a single open reading frame coding for 1,291 amino acids corresponding to a polypeptide with a molecular weight of 149,000. The amino acid sequence of the C1 toxin has a few regions highly homologous with tetanus toxin. © 1990.

引用

页码：1304 / 1311

页数：8

共 12 条

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