SYSTEMIC ISOTRETINOIN - EFFECTS ON DERMAL WOUND-HEALING IN A RABBIT EAR MODEL INVIVO

被引:26
作者
MOY, RL
MOY, LS
BENNETT, RG
ZITELLI, JA
UITTO, J
机构
[1] UNIV CALIF LOS ANGELES,MOHS MICROGRAPH SURG & CUTANEOUS ONCOL UNIT,LOS ANGELES,CA 90024
[2] UNIV SO CALIF,SCH MED,LOS ANGELES,CA 90033
[3] UNIV HLTH CTR PITTSBURGH,MONTEFIORE HOSP,DEPT MED & SURG,PITTSBURGH,PA
[4] THOMAS JEFFERSON UNIV,DEPT DERMATOL,PHILADELPHIA,PA 19107
来源
JOURNAL OF DERMATOLOGIC SURGERY AND ONCOLOGY | 1990年 / 16卷 / 12期
关键词
D O I
10.1111/j.1524-4725.1990.tb00026.x
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Clinical observations have suggested that wound healing may be altered in patients treated with systemic isotretinoin. In this study, we examined the effects of systemic isotretinoin on dermal wound healing and connective tissue metabolism in rabbit ear model. Forty 6-mm punch-biopsy wounds were created in the ears of two control rabbits as well as two experimental animals fed isotretinoin, 4 mg/kg per day. Clinical inspection and histologic examination revealed no difference between the control and isotretinoin-rabbits in terms of the time required for complete wound healing or the appearance of the final scar. The tissue removed from the wound site at days 0, 7, 14, and 21 after wounding was subjected to analysis of a collagen production and collagen gene expression. Collagen production, determined by the synthesis of ]H-3[hydroxyproline after incubation of tissue slices with ]H-3[proline in vitro of by the measurement of the steady-state levels of type I and III procollagen mRNAs, was not significantly different between the two groups. The results indicate that systemic administration of isotretinoin does not affect collagen synthesis in the rabbit ear model of wound healing.
引用
收藏
页码:1142 / 1146
页数:5
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