ANALYSIS OF THE TRANSFORMATION PRODUCTS OF DEHYDRO-L-ASCORBIC ACID BY ION-PAIRING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:69
作者
KIMOTO, E
TANAKA, H
OHMOTO, T
CHOAMI, M
机构
[1] Department of Chemistry, Faculty of Science, Fukuoka University, Fukuoka 814-01, Nanakuma, Jonan-ku
关键词
D O I
10.1006/abio.1993.1453
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Dehydro-L-ascorbic acid, the oxidation form of Lascorbic acid, converts nonenzymatically to a variety of transformation products depending upon the incubating conditions. These products, called the ascorbate cascade, possess different absorption spectra and reducing activities. Ion-pairing high-performance liquid chromatography with multiwavelength uv absorption and amperometric electrochemical detection has proven to be a useful technique for the analysis of these products. Separation and characterization in the present investigations reveal three kinds of transformation products: the polymerized intermediates of browning reaction, the reductant possessing an absorption maximum at 345 nm as the six-carbon compound, and erythro-L-ascorbic acid, 2-furoic acid, 3-hydroxy-2-pyrone, 5-methyl-3,4-dihydroxytetrone, reductic acid, and furfural as the five-carbon compound. These products possess an absorption maximum above 240 nm, and most of them are electrochemically active reducing compounds which are derived from nonreducing dehydro-L-ascorbic acid through the processes of delactonization, decarboxylation, and intramolecular rearrangement. © 1993 Academic Press, Inc.
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页码:38 / 44
页数:7
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