FUNDAMENTAL AND PRACTICAL ASPECTS OF COUPLED CAPILLARIES FOR THE CONTROL OF ELECTROOSMOTIC FLOW IN CAPILLARY ZONE ELECTROPHORESIS OF PROTEINS

被引:18
作者
NASHABEH, W [1 ]
ELRASSI, Z [1 ]
机构
[1] OKLAHOMA STATE UNIV,DEPT CHEM,STILLWATER,OK 74078
来源
JOURNAL OF CHROMATOGRAPHY | 1993年 / 632卷 / 1-2期
关键词
D O I
10.1016/0021-9673(93)80039-B
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This article represents an extension to a new approach, which was introduced very recently by our laboratory, for the control of the magnitude of electroosmotic flow in capillary zone electrophoresis. In this new approach, short fused-silica capillaries having different potentials were coupled in series and the amount of the electroosmotic flow was conveniently varied by changing the lengths of the individual capillary segments. The different coupled capillary systems evaluated in this study comprised various combinations of untreated fused-silica capillaries and polyether-coated capillaries having various electroosmotic flow characteristics. A general equation relating the average electroosmotic flow velocity in the coupled capillaries to the intrinsic electroosmotic velocity of the connected segments and their corresponding lengths has been derived and verified experimentally. The rate of the electroosmotic flow in a given system of coupled capillaries could be tuned over a range bordered by the lowest and highest intrinsic flow-rates of the connected capillary segments. In addition, a system of coupled capillaries that permitted a stepwise change in the rate of the electroosmotic flow during analysis was introduced and evaluated. These elution schemes were useful in the rapid separation of oppositely charged proteins in a single electrophoretic run and in the rapid analytical determination of the various components of heterogeneous proteins.
引用
收藏
页码:157 / 164
页数:8
相关论文
共 15 条
[1]   ELECTROPHORETIC SEPARATIONS OF PROTEINS IN CAPILLARIES WITH HYDROLYTICALLY STABLE SURFACE-STRUCTURES [J].
COBB, KA ;
DOLNIK, V ;
NOVOTNY, M .
ANALYTICAL CHEMISTRY, 1990, 62 (22) :2478-2483
[2]   ELECTROKINETIC DISPERSION IN CAPILLARY ELECTROPHORESIS [J].
DATTA, R ;
KOTAMARTHI, VR .
AICHE JOURNAL, 1990, 36 (06) :916-926
[3]  
ELRASSI Z, 1990, SEPARATION PROCESSES, P477
[4]  
GOTTSCHALK A, 1966, PROTEINS, V4, P113
[5]   MINIMIZING ADSORPTION OF PROTEINS ON FUSED-SILICA IN CAPILLARY ZONE ELECTROPHORESIS BY THE ADDITION OF ALKALI-METAL SALTS TO THE BUFFERS [J].
GREEN, JS ;
JORGENSON, JW .
JOURNAL OF CHROMATOGRAPHY, 1989, 478 (01) :63-70
[6]   ELECTROOSMOTIC FLOW-CONTROL AND MONITORING WITH AN APPLIED RADIAL VOLTAGE FOR CAPILLARY ZONE ELECTROPHORESIS [J].
HAYES, MA ;
EWING, AG .
ANALYTICAL CHEMISTRY, 1992, 64 (05) :512-516
[7]   SOME GENERAL ASPECTS OF HYDROPHOBIC INTERACTION CHROMATOGRAPHY [J].
HJERTEN, S .
JOURNAL OF CHROMATOGRAPHY, 1973, 87 (02) :325-331
[8]   FACTORS AFFECTING DIRECT CONTROL OF ELECTROOSMOSIS USING AN EXTERNAL ELECTRIC-FIELD IN CAPILLARY ELECTROPHORESIS [J].
LEE, CS ;
MCMANIGILL, D ;
WU, CT ;
PATEL, B .
ANALYTICAL CHEMISTRY, 1991, 63 (15) :1519-1523
[9]   DIRECT CONTROL OF THE ELECTROOSMOSIS IN CAPILLARY ZONE ELECTROPHORESIS BY USING AN EXTERNAL ELECTRIC-FIELD [J].
LEE, CS ;
BLANCHARD, WC ;
WU, CT .
ANALYTICAL CHEMISTRY, 1990, 62 (14) :1550-1552
[10]   CAPILLARY ZONE ELECTROPHORESIS OF PROTEINS WITH HYDROPHILIC FUSED-SILICA CAPILLARIES [J].
NASHABEH, W ;
ELRASSI, Z .
JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2) :367-383