PASSIVE TRANSFER OF ADJUVANT-INDUCED ARTHRITIS INTO IRRADIATED DA RECIPIENT RATS

被引：5

作者：

CANNON, GW

HARPER, DS

CLAYTON, F

GRIFFITHS, MM

机构：

[1] From Salt Lake City Veterans Affairs Medical Center, Division of Rheumatology, Department of Medicine, Salt Lake City, UT

[2] Department of Pathology, University of Utah, Salt Lake City, UT

来源：

AUTOIMMUNITY | 1993年 / 15卷 / 04期

关键词：

ANIMAL MODELS; ADJUVANT-INDUCED ARTHRITIS; IRRADIATION; PASSIVE TRANSFER; DA RATS;

D O I：

10.3109/08916939309115748

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

Adjuvant-induced arthritis (AIA) can be passively transferred in Dark Agouti (DA) rats by spleen and lymph node cells after culture with Concanavalin A (Con A). A model not requiring in vitro Con A expansion and activation would be important in investigations of anti-rheumatic drugs in AIA. A new model using irradiated recipients fills this need. Donor DA rats treated with 0.1 mi complete Freund's adjuvant (CFA) containing 7.5 mg M. butyricum/ml were sacrificed 11 days after CFA injection, donor spleen cells harvested, and donor spleen cells injected intravenously into recipient DA rats previously irradiated with 5 Gy. Recipient rats developed arthritis 4-14 days after spleen cell transfer. This model can now be used to further define the effects of anti-rheumatic drugs in the passive transfer of AIA by eliminating the need for the in vitro Con A-induced expansion and/or activation of donor cells.

引用

页码：267 / 274

页数：8

共 24 条

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