OF METAL-CHELATE AFFINITY-CHROMATOGRAPHY AND HYDROPHOBIC INTERACTION CHROMATOGRAPHY FOR PURIFICATION OF PLACENTAL PROTEIN-12

被引：2

作者：

BERKOVSKY, AL ^{[1
]}

POTAPOV, PP ^{[1
]}

机构：

[1] YARINVEST MED INT CO, YAROSLAVL 150062, RUSSIA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1994年 / 656卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00127-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Placental protein 12 was isolated from amniotic fluid. Albumin was removed by means of ion-exchange chromatography on DEAE-Sepharose and chromatography on Blue-Sepharose. Complete purification was obtained by metal-chelate affinity chromatography and hydrophobic interaction chromatography under mild conditions for desorption. Using this procedure large quantities of a highly purified preparation can be obtained in one run.

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页码：432 / 435

页数：4

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