DESIGN OF A HIGHLY SENSITIVE HEAT DETECTOR - ANALYSIS OF THERMAL-BEHAVIOR OF SOLUTIONS OF LYSOZYME AND BIOSYNTHETIC HUMAN GROWTH-HORMONE

被引：5

作者：

CLAUDY, P ^{[1
]}

LETOFFE, JM ^{[1
]}

BAYOL, A ^{[1
]}

BONNET, MC ^{[1
]}

机构：

[1] SANOFI ELF BIORECH,CTR LABEGE,F-31328 LABEGE,FRANCE

来源：

THERMOCHIMICA ACTA | 1990年 / 173卷

关键词：

D O I：

10.1016/0040-6031(90)80616-7

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

A new "home-made" sensor has been developed, using a Mettler TA 2000 B apparatus. A sensitivity of 0.2-mu-W was obtained. The thermal behaviour of aqueous solutions of proteins was studied. The endothermic enthalpy of transition of lysozyme has been found to be DELTA-H = + 433 +/- 20 kJ mol-1. A study of reversibility versus thermal history of the samples has been carried out. Solutions of biosynthetic human hormone H.G.H. at a concentration of 5.6 mg ml-1 have been studied. The enthalpy of transition has been determined as DELTA-H = + 181.5 +/- 13 kJ mol-1. The total irreversibility of the phenomenon has been established. No exothermal effect was observed even if a low concentration of 1 mg ml-1 was used.

引用

页码：301 / 311

页数：11

共 7 条

[1]

BEWLEY TA, 1982, INT J PEPT PROT RES, V19, P63

[2] HEAT-TRANSFER IN A DISC-TYPE DSC APPARATUS .1. EXPERIMENTAL [J].