POLYCLONAL B-CELL ACTIVATION REVEALS ANTIBODIES AGAINST HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) IN HIV-1-SERONEGATIVE INDIVIDUALS

被引:60
作者
JEHUDACOHEN, T [1 ]
SLADE, BA [1 ]
POWELL, JD [1 ]
VILLINGER, F [1 ]
DE, BR [1 ]
FOLKS, TM [1 ]
MCCLURE, HM [1 ]
SELL, KW [1 ]
AHMEDANSARI, A [1 ]
机构
[1] CTR DIS CONTROL,DIV VIRAL DIS,RETROVIRAL DIS BRANCH,ATLANTA,GA 30333
关键词
AIDS; Immune response;
D O I
10.1073/pnas.87.10.3972
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Identification of human immunodeficiency virus type 1 (HIV-1)-infected individuals is of paramount importance for the control of the spread of AIDS worldwide. Currently, the vast majority of screening centers throughout the world rely on serological techniques. As such, clinically asymptomatic but HIV-infected, seronegative individuals are rarely identified. In this report we show that 18% (30/165) of seronegative individuals who were considered to be a unique cohort of patients at high risk for HIV infection had circulating B cells that, upon in vitro polyclonal activation with pokeweed mitogen, produced antibodies reactive with HIV. Furthermore, polymerase chain reaction analysis of DNA obtained from aliquots of the peripheral blood mononuclear cells from these seronegative but pokeweed mitogen assay-positive individuals tested revealed the presence of HIV-specific sequences in a significant number of samples. In addition, depletion of CD8+ T cells from peripheral blood mononuclear cells of HIV-1-seronegative individuals prior to in vitro culture with pokeweed mitogen resulted in increased sensitivity for detecting HIV-reactive antibodies. This assay has obvious epidemiological implications, especially in the case of high-risk groups, and also provides a simple technique to enhance detection of HIV-infected individuals. Of further interest is the determination of the mechanisms related to the lack of HIV-specific antibodies in the serum of these infected individuals.
引用
收藏
页码:3972 / 3976
页数:5
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