REGULATION OF A FOS-LACZ FUSION GENE - A PARADIGM FOR QUANTITATIVE-ANALYSIS OF STIMULUS TRANSCRIPTION COUPLING

被引：89

作者：

SCHILLING, K ^{[1
]}

LUK, D ^{[1
]}

MORGAN, JI ^{[1
]}

CURRAN, T ^{[1
]}

机构：

[1] ROCHE INST MOLEC BIOL,DEPT MOLEC ONCOL & VIROL,ROCHE RES CTR,NUTLEY,NJ 07110

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 13期

关键词：

ONCOGENE; SIGNAL TRANSDUCTION; GENE REGULATION;

D O I：

10.1073/pnas.88.13.5665

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Expression of the c-fos protooncogene is induced by a great variety of extracellular stimuli. A fos-lacZ fusion gene has been constructed that recapitulates this regulation. The fos-lacZ gene was introduced into B104 neuroblastoma cells for use in a quantitative assay for stimulus-transcription coupling. Both alpha- and beta-adrenergic agonists, dibutyryl cAMP, and phorbol ester induced beta-galactosidase activity in a dose-dependent manner. Thus, the interactions of receptors with agonists and antagonists, as well as intracellular second messenger-mediated signaling events, can be analyzed quantitatively. This approach represents a prototypic method for investigating stimulus-response coupling based upon gene expression.

引用

页码：5665 / 5669

页数：5

共 35 条

[1] EXPRESSION AND PURIFICATION OF THE LEUCINE ZIPPER AND DNA-BINDING DOMAINS OF FOS AND JUN - BOTH FOS AND JUN CONTACT DNA DIRECTLY [J].

ABATE, C ;

LUK, D ;

GENTZ, R ;

RAUSCHER, FJ ;

CURRAN, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :1032-1036

[2] HUMAN PROTOONCOGENE C-JUN ENCODES A DNA-BINDING PROTEIN WITH STRUCTURAL AND FUNCTIONAL-PROPERTIES OF TRANSCRIPTION FACTOR AP-1 [J].

BOHMANN, D ;

BOS, TJ ;

ADMON, A ;

NISHIMURA, T ;

VOGT, PK ;

TJIAN, R .

SCIENCE, 1987, 238 (4832) :1386-1392

[3] BIOCHEMICAL-ANALYSIS OF TRANSCRIPTIONAL ACTIVATION BY JUN - DIFFERENTIAL ACTIVITY OF C-JUN AND V-JUN [J].

BOHMANN, D ;

TJIAN, R .

CELL, 1989, 59 (04) :709-717

[4]

CASADABAN MJ, 1983, METHOD ENZYMOL, V100, P293

[5] THE PRODUCT OF A FOS-RELATED GENE, FRA-1, BINDS COOPERATIVELY TO THE AP-1 SITE WITH JUN - TRANSCRIPTION FACTOR AP-1 IS COMPRISED OF MULTIPLE PROTEIN COMPLEXES [J].

COHEN, DR ;

FERREIRA, PCP ;

GENTZ, R ;

FRANZA, BR ;

CURRAN, T .

GENES & DEVELOPMENT, 1989, 3 (02) :173-184

[6]

CRAVEN GR, 1965, J BIOL CHEM, V240, P2468

[7] MEMORIES OF FOS [J].

CURRAN, T ;

MORGAN, JI .

BIOESSAYS, 1987, 7 (06) :255-258

[8] STRUCTURE OF THE FBJ MURINE OSTEO-SARCOMA VIRUS GENOME - MOLECULAR-CLONING OF ITS ASSOCIATED HELPER VIRUS AND THE CELLULAR HOMOLOG OF THE V-FOS GENE FROM MOUSE AND HUMAN-CELLS [J].

CURRAN, T ;

MACCONNELL, WP ;

VANSTRAATEN, F ;

VERMA, IM .

MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (05) :914-921

[9] VIRAL AND CELLULAR FOS PROTEINS ARE COMPLEXED WITH A 39,000-DALTON CELLULAR PROTEIN [J].

CURRAN, T ;

VANBEVEREN, C ;

LING, N ;

VERMA, IM .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (01) :167-172

[10] FOS AND JUN - THE AP-1 CONNECTION [J].

CURRAN, T ;

FRANZA, BR .

CELL, 1988, 55 (03) :395-397

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