A STRUCTURAL-ANALYSIS OF THE SXR REGION OF THE MOUSE Y-CHROMOSOME

被引：29

作者：

MITCHELL, MJ ^{[1
]}

BISHOP, CE ^{[1
]}

机构：

[1] INST PASTEUR,INSERM,U276,UNITE IMMUNOGENET,PARIS 15,FRANCE

来源：

GENOMICS | 1992年 / 12卷 / 01期

关键词：

D O I：

10.1016/0888-7543(92)90402-E

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three genetic functions have been mapped to the minute Sxr (sex-reversed) region of the mouse Y chromosome. These are Tdy, the primary testis determinant; Hya, the locus (either structural or regulatory) controlling the expression of the male-specific minor histocompatibility antigen H-Y; and Spy, a spermatogenic gene. Hya and Spy map to DNA deleted from the Sxr region in the deletion variant Sxrb (the ΔSxrb DNA). With the object of cloning Hya and Spy, we initiated chromosome walking in the ΔSxrb DNA. From three independent loci-Sx1, Zf2, and T5-we have isolated approximately 270 kb of ΔSxrb DNA lying in three contigs of 145, 60, and 65 kb, respectively. Within 17 kb of the 3′ end of the Zfy-2 gene, low-copy repeat elements were found in a region that extends for ∼35 kb. Probes isolated from this region detect multiple Sxr loci, some of which map to the ΔSxrb DNA present in the T5 contig DNA. Three of these multicopy probes detect ΔSxrb loci not represented in our three contigs, which means that six distinct ΔSxrb loci have now been identified. Here we present a preliminary model of the molecular structure of the DNA in this unique region. © 1992.

引用

页码：26 / 34

页数：9

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