OVEREXPRESSION OF ALCOHOL OXIDASE IN PICHIA-PASTORIS

被引：7

作者：

DEHOOP, MJ

CREGG, J

KEIZERGUNNINK, I

SJOLLEMA, K

VEENHUIS, M

AB, G

机构：

[1] UNIV GRONINGEN, BIOCHEM LAB, NIJENBORGH 16, 9747 AG GRONINGEN, NETHERLANDS

[2] UNIV GRONINGEN, ELECTRON MICROSCOPY LAB, 9751 NN GRONINGEN, NETHERLANDS

[3] OREGON GRAD INST SCI & TECHNOL, DEPT CHEM & BIOL SCI, BEAVERTON, OR 97006 USA

来源：

FEBS LETTERS | 1991年 / 291卷 / 02期

关键词：

ALCOHOL OXIDASE; METHYLOTROPHIC YEAST; PEROXISOME;

D O I：

10.1016/0014-5793(91)81306-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The protein import capacity of peroxisomes in methylotrophic yeasts was studied using Pichia pastoris containing one or two extra copies of the gene encoding the peroxisomal protein alcohol oxidase. The alcohol oxidase overproduced in this strain was only partially imported and assembled into the active, octameric form of the protein. The excess remained in the cytosol as protein aggregates composed of monomers. These results are discussed in view of the possible application of peroxisomes as storage compartments for heterologous proteins.

引用

页码：299 / 302

页数：4

共 23 条

[1] IDENTIFICATION OF CYTOSOLIC PEROXISOME PROLIFERATOR BINDING-PROTEIN AS A MEMBER OF THE HEAT-SHOCK PROTEIN HSP70 FAMILY [J].