CLONING THE REC1 GENE OF USTILAGO-MAYDIS

被引：9

作者：

HOLDEN, DW ^{[1
]}

SPANOS, A ^{[1
]}

KANUGA, N ^{[1
]}

BANKS, GR ^{[1
]}

机构：

[1] NATL INST MED RES,DIV GENET,MILL HILL,LONDON NW7 1AA,ENGLAND

来源：

CURRENT GENETICS | 1991年 / 20卷 / 1-2期

关键词：

USTILAGO; RECOMBINATION; GENE CLONING;

D O I：

10.1007/BF00312777

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The REC1 gene of Ustilago maydis plays a key role in homologous recombination and the repair of damaged DNA. In order to understand the nature and functions of the gene product, the gene has been cloned by functional complementation. A 3.8 kb cloned fragment complements the pleiotropic mitotic phenotype of different rec 1 alleles. It does not complement the UV sensitivity of two other sensitive mutants. Disruption of the chromosomal copy of the 1.566 kb open reading frame within this fragment reproduces the rec 1 pleiotropic phenotype. Furthermore, in diploids this disrupted reading frame is unable to complement previously characterised rec 1 alleles.

引用

页码：145 / 150

页数：6

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