LARGE-SCALE CELL-SEPARATION BY CENTRIFUGAL ELUTRIATION

被引：10

作者：

LUTZ, MP ^{[1
]}

GAEDICKE, G ^{[1
]}

HARTMANN, W ^{[1
]}

机构：

[1] UNIV ULM, DEPT PEDIAT, W-7900 ULM, GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 200卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90482-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Countercurrent elutriation is a powerful method for fast and gentle large-scale isolation of cells according to size and density. Current limitations of this approach are the suboptimal selectivity that is obtained and the fact that the total number of cells that can be separated per run is restricted. We describe a new separation chamber, which was calculated according to the hydrodynamic boundaries of the counterflow separation. In comparison to the chamber commonly in use, a fivefold higher cell number can be separated with this chamber at an increased separation selectivity. Furthermore the use of this chamber, which fits to the standard Beckman equipment, avoids large sampling volumes. © 1992.

引用

页码：376 / 380

页数：5

共 18 条

[1]

BERTONCELLO I, 1987, CELL SEPARATION METH, V4, P89

[2] AN IMPROVED TECHNIQUE FOR THE NEGATIVE SELECTION OF LARGE NUMBERS OF HUMAN-LYMPHOCYTES AND MONOCYTES BY COUNTERFLOW CENTRIFUGATION-ELUTRIATION [J].