PREPARATIVE LABELING OF PROTEINS WITH [S-35] METHIONINE

被引：9

作者：

BROWDER, LW

WILKES, J

RODENHISER, DI

机构：

[1] Department of Biological Sciences, University of Calgary, Calgary

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 204卷 / 01期

关键词：

D O I：

10.1016/0003-2697(92)90143-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The most common technique for preparative labeling of proteins with radioisotopes for experimental purposes utilizes 125I. This isotope has certain limitations, including the emission of γ- and X-irradiation, the release of gaseous 125I2 from solutions of Na 125I, and the potential for concentration of 125I in thyroid glands. We have discovered a means for labeling proteins rapidly and simply with [35S]methionine. The technique is applicable to a wide variety of proteins. Antibodies labeled by our technique remain functional. © 1992.

引用

页码：85 / 89

页数：5

共 7 条

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[2]

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