UNEVEN DISTRIBUTION OF INTRACELLULAR CL- IN RAT HIPPOCAMPAL-NEURONS

被引:87
作者
HARA, M [1 ]
INOUE, M [1 ]
YASUKURA, T [1 ]
OHNISHI, S [1 ]
MIKAMI, Y [1 ]
INAGAKI, C [1 ]
机构
[1] KANSAI MED UNIV,DEPT PHARMACOL,OSAKA,JAPAN
关键词
LOCAL INTRACELLULAR CL-CONCENTRATION; HIPPOCAMPAL NEURON; ETHACRYNIC ACID; FUROSEMIDE; BUMETANIDE; CL-PUMP; NA+/K+/2CL COTRANSPORTER;
D O I
10.1016/0304-3940(92)90250-B
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Electrophysiological observations of neurons suggest that perikarya and dendrites differ in local intracellular Cl-concentration ([Cl-]i), that has not been demonstrated yet. Regional [Cl-]i in cultured hippocampal neurons was estimated using a Cl-sensitive fluorescent dye. Calibration showed that perikaryonic [Cl-]i was lower than dendritic [Cl-]i. Ethacrynic acid, an inhibitor of the outwardly directed Cl-pump, increased the perikaryonic but not dendritic [Cl-]i. A decrease in [Cl-]i induced by furosemide or bumetanide, inhibitors of Na+/K+/2Cl- cotransporters, was more prominent in dendrites than in perikaria. These findings suggest that uneven distribution of Cl- is generated by the region-specific localization of these transporters.
引用
收藏
页码:135 / 138
页数:4
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