DISTRIBUTION AND IMMUNOCHEMICAL SPECIFICITIES OF FIMBRIAE OF PORPHYROMONAS-GINGIVALIS AND RELATED BACTERIAL SPECIES

被引：34

作者：

OGAWA, T ^{[1
]}

MUKAI, T ^{[1
]}

SHIMAUCHI, H ^{[1
]}

TODA, Y ^{[1
]}

HAMADA, S ^{[1
]}

机构：

[1] OSAKA UNIV,FAC DENT,DEPT ORAL MICROBIOL,SUITA,OSAKA 565,JAPAN

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 6卷 / 06期

关键词：

PORPHYROMONAS-GINGIVALIS; FIMBRIAE; PERIODONTAL DISEASE; IMMUNOCHEMISTRY;

D O I：

10.1111/j.1399-302X.1991.tb00504.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Rabbit polyclonal antibody (Poly-1) and mouse monoclonal antibodies (mAbs) TO-11, TO-14 and TO-M1 specific for Porphyromonas gingivalis 381 fimbriae were prepared. Poly-1 and the 3 mAbs were screened for their reactivity with whole cells of oral and nonoral black-pigmented bacterial species by enzyme-linked immunosorbent assay (ELISA) and the binding experiment using [I-125] Poly-1 and [I-125] mAbs. ELISA revealed that Poly-1 definitely reacted with whole cells of all the 11 strains of P. gingivalis tested. However, 8 of 11 P. gingivalis strains reacted with mAbs TO-11, TO-14 and TO-M1. These results were confirmed by the specific binding of radiolabelled Poly-1 and mAb TO-11 to the 8 strains. The M(r) of the fimbrial subunit protein (fimbrilin) isolated and purified from P. gingivalis strains 381, BH18/10, HW24D-1, 6/26 and OMZ 314 was 41 kDa by sodium dodecylsulfate-polyacrylamide gel electrophoresis. It was found by immunoblotting that mAbs TO-11 and TO-14/TO-M1 recognized different epitopes of fimbrial protein from P. gingivalis strains. Immunoelectron micrographs of whole cells and the purified fimbriae of P. gingivalis strains visualized similar serotype-specific antibody bindings to the fimbriae. These results indicate that 11 strains of P. gingivalis could be divided into at least 2 separate groups based on the immunochemical specificities of the fimbriae.

引用

页码：332 / 340

页数：9

共 35 条

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