UPTAKE OF ORGANIC-ANIONS BY HEPATOCYTE MONOLAYERS - CODIFFUSION VERSUS FACILITATED DISSOCIATION

被引：4

作者：

BURCZYNSKI, FJ ^{[1
]}

MORAN, JB ^{[1
]}

CAI, ZS ^{[1
]}

机构：

[1] UNIV MISSOURI, SCH MED, DEPT INTERNAL MED, COLUMBIA, MO 65212 USA

来源：

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | 1993年 / 71卷 / 12期

关键词：

HEPATOCYTES; ALBUMIN; DIFFUSION; CLEARANCE; PALMITATE;

D O I：

10.1139/y93-130

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We used the clearance ratio test to determine whether the presence of albumin enhances the uptake of palmitate by hepatocyte monolayers. If uptake is facilitated, then the ratio of palmitate clearance by hepatocytes in the presence of 600 and 300 mu M albumin (i.e. Cl-t600/Cl-t300) will exceed the square root of the ratio of the unbound fractions at 600 and 300 mu M albumin (i.e., root alpha(600)/alpha(300)). The latter value was determined using the heptane-water partitioning technique. The hepatocyte clearance ratio (0.92 +/- 0.06) exceeded the predicted value (0.80) by 15% (p < 0.05). The observed clearance rate also exceeded that predicted by the diffusion-reaction model.

引用

页码：863 / 867

页数：5

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