PREPARATION OF STABLE ENZYME-COENZYME COMPLEXES WITH ENDOGENOUS CATALYTIC ACTIVITY

被引：6

作者：

GACESA, P

VENN, RF

机构：

来源：

BIOCHEMICAL JOURNAL | 1979年 / 177卷 / 01期

关键词：

D O I：

10.1042/bj1770369

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N6-(6-Aminohexylcarbamoylmethyl)-NAD+ was coupled to lactate dehydrogenase by using glutaraldehyde to form an active complex. The stability of this complex could be considerably improved by reduction with KBH4, although this treatment caused a partial decrease in specific activity. NAD+ was also coupled directly to the enzyme by this method. All of these complexes exhibited an intrinsic activity in the absence of exogenous NAD+.

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页码：369 / 372

页数：4

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