CELLULASE PRODUCTION AND ACTIVITY IN A SPECIES OF CLADOSPORIUM

被引:11
作者
ABRHA, B [1 ]
GASHE, BA [1 ]
机构
[1] UNIV ADDIS ABABA,DEPT BIOL,POB 1176,ADDIS ABABA,ETHIOPIA
关键词
ASSAY PH AND TEMPERATURE; CARBON AND NITROGEN SOURCES; CELLULASE; CLADOSPORIUM; SURFACTANTS;
D O I
10.1007/BF01195839
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A Cladosporium species produced large amounts of cellulase enzyme components when grown in shake-culture with medium containing carboxymethylcellulose. There was significantly less activity when Avicel, filter paper or cotton were used as substrates. KNO3 was better than NH4Cl or urea for the production of cellulase. Tween 80 at 0.1% (w/v) increased the production of cellulase by 1.5 to 4.5-fold. All the cellulase components were optimally active in the assay at pH .5.0 and 60-degrees-C.
引用
收藏
页码:164 / 166
页数:3
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