CRYSTALLIZATION OF AND PRELIMINARY-X-RAY DATA FOR THE NEGATIVE REGULATOR (AMIC) OF THE AMIDASE OPERON OF PSEUDOMONAS-AERUGINOSA

被引：12

作者：

WILSON, SA

CHAYEN, NE

HEMMINGS, AM

DREW, RE

PEARL, LH

机构：

[1] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOLEC BIOL,BIOMOLEC STRUCT & MODELLING UNIT,GOWER ST,LONDON WC1E 6BT,ENGLAND

[2] IMPERIAL COLL SCI TECHNOL & MED,BLACKETT LAB,BIOPHYS SECT,LONDON SW7 2BZ,ENGLAND

[3] UNIV LONDON BIRKBECK COLL,DEPT CRYSTALLOG,MOLEC BIOL LAB,LONDON WC1E 7HX,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 222卷 / 04期

基金：

英国惠康基金;

关键词：

PSEUDOMONAS-AERUGINOSA; NEGATIVE REGULATOR; AMIDASE; CATABOLIC OPERON; ANTI-TERMINATION MECHANISM;

D O I：

10.1016/0022-2836(91)90579-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P42212 (a = 104·4 A ̊, c = 66·6 A ̊) with one subunit in the asymmetric unit. Crystals diffract beyond 2·8 Å. © 1991.

引用

页码：869 / 871

页数：3

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