DETERMINATION OF COCAETHYLENE, COCAINE AND THEIR METABOLITES IN RAT SERUM MICROSAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, AND ITS APPLICATION TO PHARMACOKINETIC STUDIES IN RODENTS

被引：22

作者：

LAU, CE

机构：

[1] Department of Psychology, Rutgers University, New Brunswick

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 582卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(92)80315-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A single-solvent extraction step high-performance liquid chromatographic method is described for quantitating cocaethylene in rat serum microsamples (50 mul), a substance formed in vivo when cocaine and ethanol are present concurrently. The separation used a 2 mm I.D. reversed-phase Nova-Pak C18 column with a mobile phase of acetonitrile-phosphate buffer containing an ion-pairing reagent. With an ultraviolet detector operated at 230 nm, a linear response was observed from 0.05 to 2.0 mug/ml with a detection limit of 5 ng/ml for cocaethylene, cocaine and norcocaine. The method showed a longer half-life for cocaethylene than for cocaine in rat.

引用

页码：167 / 172

页数：6

共 26 条

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