HETEROLOGOUS EXPRESSION OF RECOMBINANT HUMAN GLUTATHIONE TRANSFERASE-A1-1 FROM A HEPATOMA-CELL LINE

被引：64

作者：

STENBERG, G ^{[1
]}

BJORNESTEDT, R ^{[1
]}

MANNERVIK, B ^{[1
]}

机构：

[1] UNIV UPPSALA, CTR BIOMED, DEPT BIOCHEM, BOX 576, S-75123 UPPSALA, SWEDEN

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1992年 / 3卷 / 01期

基金：

英国工程与自然科学研究理事会; 加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/1046-5928(92)90060-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone, λGTHA1, encoding human glutathione transferase A1-1 has been isolated from a hepatoma HepG2 cDNA library. At the nucleotide level, the new clone showed minor differences from cDNA deriving from normal liver, but the deduced amino acid sequence was identical to the structure previously described. The protein was expressed from a plasmid, pKHA1, and isolated by a single-step affinity purification on an S-hexylglutathione Sepharose matrix. The yield of the recombinant protein was 165 mg from a 3-liter culture of bacteria. © 1992 Academic Press, Inc.

引用

页码：80 / 84

页数：5

共 32 条

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