EFFECTIVE METHOD FOR SYNTHETIC PEPTIDE IMMOBILIZATION THAT INCREASES THE SENSITIVITY AND SPECIFICITY OF ELISA PROCEDURES

被引：29

作者：

IVANOV, VS ^{[1
]}

SUVOROVA, ZK ^{[1
]}

TCHIKIN, LD ^{[1
]}

KOZHICH, AT ^{[1
]}

IVANOV, VT ^{[1
]}

机构：

[1] CENT INST EPIDEMIOL,MOSCOW 111123,USSR

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 153卷 / 1-2期

关键词：

STREPTAVIDIN-BIOTIN INTERACTION; BIOTINYLATED PEPTIDE; PEPTIDE-BASED ELISA; HIV-1/2; AIDS;

D O I：

10.1016/0022-1759(92)90326-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is described for the immobilization of synthetic peptide antigens on a plastic solid phase for performing ELISA. The use of a streptavidin-biotinylated peptide system for coating microplates with peptide antigen markedly increased both the sensitivity and the specificity compared to a standard ELISA based on synthetic peptides. The procedure was used for the detection of HIV-1-specific antibodies.

引用

页码：229 / 233

页数：5

共 11 条

[1]

Engvall E, 1980, Methods Enzymol, V70, P419

[2] DIAGNOSIS OF AIDS BY USING A 12-AMINO ACID PEPTIDE REPRESENTING AN IMMUNODOMINANT EPITOPE OF THE HUMAN-IMMUNODEFICIENCY-VIRUS [J].