An Homogeneous Radioimmunoassay for Abscisic Acid using a Scintillation Proximity Assay Technique

被引：16

作者：

Whitford, P. N. ^{[1
]}

Croker, S. J. ^{[1
]}

机构：

[1] Univ Bristol, Long Ashton Res Stn, AFRC, Dept Agr Sci,Inst Arable Crops Res, Bristol BS18 9AF, Avon, England

来源：

PHYTOCHEMICAL ANALYSIS | 1991年 / 2卷 / 03期

关键词：

Radioimmunoassay; abscisic acid; scintillation proximity assay;

D O I：

10.1002/pca.2800020309

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An homogeneous radioimmunoassay (RIA) for the plant growth regulator abscisic acid (ABA) has been developed by modifying an established heterogeneous assay. The introduction of a scintillation proximity reagent has enabled the production of a one-step RIA in which radioactivity associated with antibody-bound ABA can be counted in the presence of the unbound radiolabelled ABA and without the addition of a liquid scintillation cocktail. The assay has been validated by comparing the results with those obtained by combined gas chromatography/mass spectrometry and the precision of each method determined. The homogeneous RIA has a working range of 0.165-13.2 ng ABA and has been used to determine ABA concentration in extracts of leaves of tomato, Lycopersicon esculentum Mill. cv. Ailsa Craig (wild type) and three wilty mutants, notabilis, flacca and sitiens.

引用

页码：134 / 136

页数：3

共 11 条

[1] SCINTILLATION PROXIMITY ASSAY [J].